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廖焕琴, 钟敏艳, 徐放, 杨会肖, 杨晓慧, 陈新宇, 张卫华, 潘文, 徐斌
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广东省林科院
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摘要: |
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试验以朱顶红（Hippeastrum rutilum）无菌苗为材料，通过筛选基本培养基、低 pH 水培、添
加不同种类和浓度的凝固剂、不同浓度蔗糖、不同褐化剂来探讨鳞茎组织培养中抑制褐化的有效方法。
结果表明：朱顶兰在 MS 基本培养基中生长状况较好，褐化程度最低；低 pH 水培显著影响朱顶红的生
长与增殖，且不能有效抑制朱顶红鳞茎褐化；以琼脂粉 A 为凝固剂，半固体状态，附加 30 g · L-1 蔗糖的
MS 培养基有利于朱顶红鳞茎的抗褐化和增殖；在继代培养基中添加 1.0 g · L-1 活性炭可有效抗褐化。因
此，朱顶红鳞茎继代过程中适宜的培养条件为：MS+2.0 mg · L-1 6-BA +30 g · L-1 蔗糖 +1.8 g · L-1 琼脂粉 A 或 MS+2.0 mg · L-1 6-BA +30 g · L-1 蔗糖 +6.0 g · L-1 卡拉胶 +1.0 g · L-1 活性炭。
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关键词:
朱顶红；鳞茎；组织培养；褐化；增殖率
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DOI：
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分类号：
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基金项目： |
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Studies on Anti-browning in Bulbs Tissue Culture of Hippeastrum rutilum |
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Liao Huanqin, Xu Bin
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Guangdong Academy of Forestry
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Abstract: |
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The effective approaches of anti-browning in bulbs tissue culture was studied with the aseptic
seedling of Hippeastrum rutilum by selecting basic medium, low pH ydroponic culture, adding different type and
concentration of coagulant, different concentration of sucrose, different anti-browning agent. MS medium showed
better growth status and lower rate of browning. Low pH hydroponic culture significantly affected the growth
status, and could not effectively inhibit the browning of bulb. MS medium with agar powder A (semi-solid state)
and sucrose with concentration of 30 g · L-1 was beneficial to the browning resistance and proliferation. Adding
1.0 g · L-1 activated carbon to the medium can effectively prevent browning. Therefore, the suitable tissue culture
conditions for bulbs of H. rutilum were MS + 2.0 mg · L-1 6-BA + 30 g · L-1 sucrose + 1.8 g · L-1 agar powder A or
MS + 2.0 mg · L-1 6-BA + 30 g · L-1 sucrose + 6.0 g · L-1 carrageenan + 1.0 g · L-1 activated carbon.
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Key words:
Key words Hippeastrum rutilum；bulb ；tissue culture；browning；proliferation rate
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