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Through single-factor test, concentrations of the main factors influencing SRAP-PCR reaction(Mg2+, dNTP, primer and Taq DNA polymerase, respectively ) in two pine hybrids were optimized. The optimized reaction system was that a total 25ul containing 1×PCR Buffer, Mg2+ 2.0 mmol/L, dNTPs 0.2 5mmol/L, Taq DNA polymerase 1.0U, primer 0.4 umol/L, template DNA 30-50ng and 1% DMSO. Using this optimized system, SRAP-PCR reactions were performed on two hybrid pines(F1 of slash pine×Caribbean pine and loblolly pine×Caribbean pine), and scorable bands were amplified. This study laid a solid foundation for further researches on SRAP markers in hybrid pines.
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